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Updated: Apr 28, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
Published on: August 16, 2015
Cell cycle synchronization and flow cytometry analysis of mammalian cells
Naoko Yoshizawa-Sugata1, Hisao Masai
1Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
Abstract:
Analysis of cellular DNA content and measurement of pulse-labeled newly replicated DNA by flow cytometry are useful techniques for cell cycle studies. In this chapter, we describe the protocols for cell cycle synchronization of mammalian cells, including time course designs and consideration of cell types to achieve successful experiments, along with the methods for detection of DNA. Some selected applications dealing with siRNA-mediated knockdown are also presented.

