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Updated: Apr 28, 2026
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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Published on: September 20, 2019
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Live-cell fluorescence imaging for phenotypic analysis of mitosis
Sushama Sivakumar1, John R Daum, Gary J Gorbsky
1Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, MS 48, Oklahoma City, OK, 73104, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2014
Summary
Live-cell fluorescence microscopy helps study cell division defects. This guide offers strategies for clear imaging of mammalian cell mitosis using standard equipment.
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- Live-cell fluorescence microscopy is crucial for identifying abnormal cell division (mitotic phenotypes).
- Challenges include maintaining cell health during imaging while achieving necessary resolution.
Purpose of the Study:
- To present strategies for monitoring and analyzing mammalian cell mitosis.
- To enable detailed study of mitotic progression using widely available microscopy systems.
Main Methods:
- Utilizing standard, inverted fluorescence microscopy systems.
- Implementing specific strategies for live imaging during cell mitosis.
Main Results:
- Successful monitoring and analysis of mammalian cell mitosis.
- Characterization of aberrant mitotic phenotypes.
Conclusions:
- Standard fluorescence microscopy can effectively image cell mitosis.
- The described strategies facilitate robust analysis of cell division processes.
