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Published on: August 14, 2019
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Simultaneous determination of eight flavonoids in propolis using chemometrics-assisted high performance liquid
Yan-Mei Sun1, Hai-Long Wu1, Jian-Yao Wang1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Summary
A new HPLC-DAD method using alternating trilinear decomposition (ATLD) accurately quantifies eight flavonoids in propolis. This efficient technique resolves overlapping peaks, offering a reliable alternative for complex sample analysis.
Area of Science:
- Analytical Chemistry
- Chromatography
- Spectroscopy
Background:
- Simultaneous determination of multiple flavonoids in complex matrices like propolis is challenging due to peak overlaps.
- High-performance liquid chromatography coupled with diode array detection (HPLC-DAD) is a common technique, but often requires advanced data processing for resolution.
- Second-order calibration methods offer improved selectivity and sensitivity for resolving co-eluting analytes.
Purpose of the Study:
- To develop and validate a fast analytical strategy for simultaneous determination of eight flavonoids in propolis capsules.
- To utilize the alternating trilinear decomposition (ATLD) algorithm to overcome peak overlapping issues in HPLC-DAD data.
- To provide an efficient, sensitive, and cost-effective method for accurate flavonoid quantification.
Main Methods:
- High-performance liquid chromatography (HPLC) with a C18 column was used for separation.
- Ultrasound-assisted extraction with methanol was employed for sample preparation.
- Alternating trilinear decomposition (ATLD) algorithm was applied to second-order calibration data from HPLC-DAD to resolve overlapping peaks and quantify analytes.
- Method validation included linearity, limit of detection (LOD), accuracy (recovery), and prediction error (RMSEP).
Main Results:
- The method achieved a chromatographic separation within 13 minutes.
- Good linearity was observed with correlation coefficients (r) ≥ 0.9947.
- Low limits of detection (LODs) ranged from 3.39-33.05 ng/mL.
- Accuracy was confirmed with recoveries between 91.9% and 110.2%, and RMSEP < 1.1 μg/mL.
- The ATLD-assisted HPLC-DAD method successfully resolved and quantified eight flavonoids in propolis capsules, even with interferences.
Conclusions:
- The developed ATLD-assisted HPLC-DAD method is efficient, sensitive, and cost-effective for analyzing flavonoids.
- This approach provides an alternative for accurate analyte quantification, especially when complete chromatographic separation is difficult.
- The method was successfully applied to propolis capsules, yielding satisfactory results.
