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Updated: Apr 28, 2026
Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
Published on: April 5, 2015
Identification of constitutively active AtMPK6 mutants using a functional screen in Saccharomyces cerevisiae
Elodie Hudik1, Souha Berriri, Heribert Hirt
1Institut de Biologie des Plantes, UMR CNRS-8618, Centre National De La Recherche Scientifique, Rue de Noetzlin, Bâtiment 630, 91405, Orsay Cedex, France.
Abstract:
MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional expression assay in yeast.
