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Published on: March 8, 2012
Next-generation sequencing analysis for detecting human papillomavirus in oral verrucous carcinoma
Manar Samman1, Henry Wood1, Caroline Conway1
1Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, United Kingdom.
Objective:
The etiology of oral verrucous carcinoma is unknown, and human papillomavirus 'involvement' remains contentious. The uncertainty can be attributed to varied detection procedures and difficulties in defining 'gold-standard' histologic criteria for diagnosing 'verrucous' lesions. Their paucity also hampers investigation. We aimed to analyze oral verrucous lesions for human papillomavirus (HPV) subtype genomes.
Study Design:
We used next-generation sequencing for the detection of papillomavirus sequences, identifying subtypes and computing viral loads. We identified a total of 78 oral verrucous cases (62 carcinomas and 16 hyperplasias). DNA was extracted from all and sequenced at a coverage between 2.5% and 13%.
Results:
An HPV-16 sequence was detected in 1 carcinoma and 1 hyperplasia, and an HPV-2 sequence was detected in 1 carcinoma out of the 78 cases, with viral loads of 2.24, 8.16, and 0.33 viral genomes per cell, respectively.
Conclusions:
Our results indicate no conclusive human papillomavirus involvement in oral verrucous carcinoma or hyperplasia.
