Pharmacological tools for hydrogen sulphide research: a brief, introductory guide for beginners

The purpose of this brief review is to help researchers in their initial approach to the H2S field and to provide answers for the most frequently posed questions by newcomers to the topic related to H2S donors and inhibitors of H2S synthesis, as well as methods to measure H2S production. Here the reader will find a practical guide that provides fast and to the point information on how to (i) deliver H2S to cells; (ii) modulate its endogenous production; and (iii) measure its levels in fluids, cells and tissues in order to gain an understanding of its role in health and disease.