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Updated: Apr 28, 2026
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
Published on: June 9, 2023
Ultrasensitive enzyme-free immunoassay for squamous cell carcinoma antigen using carbon supported Pd-Au as
Jian Gao1, Bin Du1, Xiaoyue Zhang1
1Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Abstract:
A novel nonenzymatic sandwich-type electrochemical immunosensor has been developed to detect squamous cell carcinoma antigen (SCCA). Nitrogen-doped graphene sheet (N-GS) was used to increase capacity of capturing primary antibodies (Ab1). Carbon-supported Pd-Au binary nanoparticles (Pd-Au/C) were synthesized and used to label secondary antibodies (Ab2). The specific binding of SCCA and antibodies enabled a quantitative attachment of Pd-Au/C on the electrode surface. Electrocatalytic analysis showed that the prepared Pd-Au/C exhibit excellent electrocatalytic activity towards hydrogen peroxide (H2O2). We use current response of electrocatalytic labels Pd-Au/C to detect the concentration of SCCA. The unique nonenzymatic immunosensor exhibits a relatively wide linear range from 0.005 to 2 ng mL(-1) and high sensitivity with a low detection limit of 1.7 pg mL(-1). The immunsensor also shows good reproducibility (4.2%) and stability (5.8%), which makes it an enormous application prospect in clinical research.
