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Class I ADP-ribosylation factors are involved in enterovirus 71 replication

Jianmin Wang1, Jiang Du1, Qi Jin1

  • 1MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Plos One
|June 10, 2014
PubMed

Insights

Enterovirus 71 replication, a cause of hand, foot, and mouth disease, relies on host secretory pathways. Targeting GBF1 and ADP-ribosylation factors offers a potential antiviral strategy.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Enterovirus 71 (EV71) causes hand, foot, and mouth disease in children.
  • EV71 replication occurs in unique cytoplasmic compartments.
  • The cellular pathways hijacked by EV71 to form these compartments are unknown.

Purpose of the Study:

  • Investigate the role of the cellular secretory pathway in EV71 replication.
  • Identify specific host factors essential for EV71 replication.

Main Methods:

  • Loss-of-function assays using small interfering RNA (siRNA).
  • Depletion of specific ADP-ribosylation factors (ARFs).
  • Assessment of brefeldin-A-sensitive guanidine nucleotide exchange factor GBF1 activity.

Main Results:

  • EV71 RNA replication is dependent on Class I ADP-ribosylation factors.
  • Depleting ARF1 and ARF3 significantly inhibited EV71 replication.
  • GBF1 was found to be critical for EV71 replication.

Conclusions:

  • EV71 replication is linked to the cellular secretory pathway.
  • GBF1-mediated activation of Class I ARFs is essential for EV71 replication.
  • The secretory pathway represents a potential target for novel antiviral therapies against EV71.

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