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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Related Experiment Video

Updated: Apr 28, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Mapping-by-sequencing accelerates forward genetics in barley.

Martin Mascher, Matthias Jost, Joel-Elias Kuon

|June 12, 2014

Summary

This summary is machine-generated.

Mapping-by-sequencing is a powerful genetic mapping tool. This study shows its utility in barley using exome sequencing, overcoming challenges with incomplete reference genomes.

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Last Updated: Apr 28, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

BEST: Barcode Enabled Sequencing of Tetrads

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Published on: May 1, 2014

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Area of Science:

Plant genetics
Genomic analysis
Molecular biology

Background:

Mapping-by-sequencing is a valuable genetic mapping technique.
Its application is limited in species with incomplete reference genomes.
Barley genetics presents unique challenges due to fragmented sequence resources.

Purpose of the Study:

To adapt and apply mapping-by-sequencing to barley, a complex plant genome.
To identify genes controlling leaf initiation rate using this method.
To demonstrate the feasibility of exome resequencing for genetic mapping in barley.

Main Methods:

Exome sequencing of phenotypic bulks from a barley mapping population.
Read depth analysis to identify candidate genes.
Confirmation of candidate genes using independent mutant alleles.

Main Results:

Successfully applied mapping-by-sequencing in barley.
Identified a candidate gene associated with increased leaf initiation rate.
Validated the candidate gene with independent mutant alleles.

Conclusions:

Exome resequencing is effective for genetic mapping in barley.
Genomic resources and exome sequencing can overcome challenges in complex plant genomes.
This approach facilitates gene discovery in species with limited genomic data.