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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
Published on: September 26, 2025
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Unidirectional P-body transport during the yeast cell cycle
Cecilia Garmendia-Torres1, Alexander Skupin2, Sean A Michael3
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
Plos One
|June 12, 2014
Summary
P-bodies (PB) are dynamic RNA granules crucial for gene regulation. In yeast cell division, PBs move unidirectionally to daughter cells, facilitated by the Myo4p/She2p machinery, impacting growth.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- P-bodies (PB) are conserved RNA granules involved in post-transcriptional gene regulation.
- RNA granules facilitate RNA transport in various biological processes, including development and neuronal plasticity.
- Understanding PB dynamics is crucial for comprehending cellular organization and function.
Purpose of the Study:
- To investigate the dynamics of P-bodies during yeast cell division.
- To elucidate the mechanisms and directionality of PB transport.
- To determine the role of PBs in daughter cell growth.
Main Methods:
- Microfluidic-based fluorescent microscopy of single yeast cells.
- Automated image analysis to track PB movement.
- Single-cell analysis of cell size under nutrient-limiting conditions.
Main Results:
- P-bodies exhibit unidirectional transport from mother to daughter cells during mitosis.
- PBs show constrained hovering near the bud site before bud emergence.
- Myo4p/She2p RNA transport machinery is essential for these PB behaviors.
- PBs appear to play a significant role in daughter cell growth under nutrient limitation.
Conclusions:
- P-body dynamics are tightly regulated during cell division, involving directed transport.
- The Myo4p/She2p pathway mediates PB movement, highlighting its importance in cellular asymmetry.
- PBs contribute to daughter cell fitness, particularly under stress conditions.
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