Related Experiment Video
Updated: Jul 5, 2026
07:55
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
Published on: May 21, 2020
CYClones: A highly powered, fully genotyped, 8-parent yeast mapping population.
Gareth A Cromie1, Russell S Lo1, Trey S Morgan1
1Pacific Northwest Research Institute, Seattle, Washington 98122, USA.
G3 (Bethesda, Md.)
|July 3, 2026
Summary
Researchers created CYClones, a large library of yeast segregants, to study genetic diversity and complex traits. This resource aids in dissecting the genetic basis of variations in Saccharomyces cerevisiae.
Area of Science:
- Genetics
- Yeast Biology
- Population Genetics
Background:
- Saccharomyces cerevisiae exhibits significant genetic diversity across natural isolates.
- Understanding this diversity is key to dissecting complex traits.
- Previous resources have not fully captured the global genetic variation in yeast.
Purpose of the Study:
- To construct and characterize CYClones, a comprehensive library of yeast segregants.
- To enable high-resolution genetic dissection of complex traits in Saccharomyces cerevisiae.
- To provide a community resource for exploring yeast genetic architecture.
Main Methods:
- Generated 11,392 segregants from a multiparental cross of eight diverse S. cerevisiae strains.
- Imputed whole-genome sequences for all segregants.
- Characterized haplotype representation and genetic diversity captured by the library.
Main Results:
- CYClones captures a substantial fraction of global S. cerevisiae genetic diversity.
- Haplotype representation is well-maintained across the genome.
- The library provides high power (≥95%) for detecting variants with low heritability and fine mapping resolution.
Conclusions:
- CYClones is a powerful resource for dissecting the genetic architecture of complex and quantitative traits in yeast.
- It facilitates the identification of causal variants underlying phenotypic diversity.
- This resource supports research into context-dependent mutational effects in Saccharomyces cerevisiae.
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