Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

1.3K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.3K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.9K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting amyloid positivity in patients with mild cognitive impairment using a radiomics approach.

Scientific reports·2021
Same author

Impact of acute kidney injury on survival in patients with chronic hepatitis C: a retrospective cohort study.

BMC infectious diseases·2021
Same author

Amyloid Positivity in the Alzheimer/Subcortical-Vascular Spectrum.

Neurology·2021
Same author

Comparison of Longitudinal Changes of Cerebral Small Vessel Disease Markers and Cognitive Function Between Subcortical Vascular Mild Cognitive Impairment With and Without <i>NOTCH3</i> Variant: A 5-Year Follow-Up Study.

Frontiers in neurology·2021
Same author

Prediction of tau accumulation in prodromal Alzheimer's disease using an ensemble machine learning approach.

Scientific reports·2021
Same author

Author Correction: Concordance in detecting amyloid positivity between <sup>18</sup>F-florbetaben and <sup>18</sup>F-flutemetamol amyloid PET using quantitative and qualitative assessments.

Scientific reports·2021
Same journal

Concise Synthesis of Pyrano/Benzopyrano[2,3-<i>c</i>]pyrazol-6-ones via Ir/Rh-Catalyzed C-H Activation and Tandem Annulation.

The Journal of organic chemistry·2026
Same journal

Regiodivergent Cyclizations of Donor-Acceptor Cyclopropanes with Hydrazines under Solventless, Mechanochemical Milling.

The Journal of organic chemistry·2026
Same journal

Photocatalytic Decarboxylative Coupling of Diacyl Peroxides with TEMPO.

The Journal of organic chemistry·2026
Same journal

Mechanistic Insights into the Isothiourea-Catalyzed Addition Reaction of a <i>para</i>-Nitrophenyl Ester and an <i>N</i>-Benzylpyridinium Salt.

The Journal of organic chemistry·2026
Same journal

Catalytic, Regioselective Hydrocarbofunctionalization of Unactivated Alkenes with Anthrones and 9-Phenanthrols.

The Journal of organic chemistry·2026
Same journal

Copper(II)-Catalyzed <i>N</i>-Arylation and <i>N</i>-Vinylation of Oxadiazolones with Boronic Acids under Mild Conditions.

The Journal of organic chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 28, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

11.2K

Phosphorescent sensor for phosphorylated peptides based on an iridium complex.

Jung Hyun Kang1, Hee Jin Kim, Tae-Hyuk Kwon

  • 1Department of Chemistry, College of Natural Sciences, Seoul National University , Seoul 151-747, South Korea.

The Journal of Organic Chemistry
|June 13, 2014
PubMed
Summary
This summary is machine-generated.

A novel iridium-based sensor detects phosphorylated peptides by increasing luminescence with higher negative charges. This sensor offers visual discrimination from other molecules, aiding cellular mechanism studies.

More Related Videos

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
07:45

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Published on: June 6, 2022

2.3K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

8.5K

Related Experiment Videos

Last Updated: Apr 28, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

11.2K
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
07:45

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Published on: June 6, 2022

2.3K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

8.5K

Area of Science:

  • Coordination Chemistry
  • Chemical Sensing
  • Biomolecular Detection

Background:

  • Phosphorylated peptides play crucial roles in cellular signaling pathways.
  • Developing selective and sensitive sensors for these molecules is essential for biological research.
  • Existing fluorescent sensors may lack specificity or visual detection capabilities.

Purpose of the Study:

  • To develop a highly sensitive and selective phosphorescent sensor for phosphorylated peptides.
  • To investigate the sensor's response to varying degrees of peptide phosphorylation.
  • To compare the sensor's performance against a fluorescent control sensor.

Main Methods:

  • Synthesis of a bis[(4,6-difluorophenyl)pyridinato-N,C(2')]iridium(III) picolinate (FIrpic) derivative coupled with bis(Zn(2+)-dipicolylamine) (ZnDPA) as sensor 1.
  • Preparation of an anthracene-based fluorescent sensor (sensor 2) as a control.
  • Spectroscopic analysis of sensor responses to phosphorylated peptides, neutral peptides, and various anions.

Main Results:

  • Sensor 1 exhibited a gradual increase in emission intensity (up to 16-fold) with increasing negative charge (-2, -4, -6) of phosphorylated peptides.
  • Sensor 1 showed minimal response to neutral peptides, non-phosphorylated peptides, and common anions.
  • Sensor 1 enabled visual discrimination between phosphorylated peptides and adenosine triphosphate under UV-vis light, unlike sensor 2.

Conclusions:

  • The FIrpic-ZnDPA sensor demonstrates high selectivity and sensitivity for phosphorylated peptides based on charge.
  • The sensor's enhanced phosphorescence upon binding is linked to reduced ion repulsion and charge transfer characteristics.
  • This phosphorescent sensor provides a valuable tool for detecting and visualizing phosphorylated peptides in biological contexts.