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Updated: Apr 28, 2026
Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
Published on: September 3, 2021
Detection of liver micrometastases from colorectal origin by perfusion CT in a rat model
Caroline Hatwell1, Magaly Zappa, Mathilde Wagner
1Department of Colorectal Surgery, Beaujon Hospital (AP-HP), 100 boulevard du General Leclerc, 92118 Clichy, Paris, France; Research Unit Bichat-Beaujon, INSERM U773, Universite Paris VII (Denis Diderot), Paris, France. yves.panis@bjn.aphp.fr.
Background:
Some patients with colorectal carcinoma have liver metastases (LMs) which cannot be detected by conventional imaging. This study aimed to assess whether hepatic perfusion changes induced by micrometastases can be detected by perfusion computed tomography (CT).
Methods:
LMs were produced in rats by injecting carcinoma cells into the portal vein. Perfusion CT was performed at microscopic (day 10), interval (day 17), and macroscopic stage (day 34). Perfusion parameters were computed using a dual-input one-compartmental model.
Results:
Micro and macro LMs presented a mean diameter of 0.5 and 2.6 mm, respectively. Compared to controls, LMs at interval (1.1 mm) and macroscopic stage induced significant perfusion changes: a decrease of 42% (P=0.004) and 41% (P=0.029) in hepatic transit time and an increase of 292% (P=0.073) and 240% (P=0.001) in portal delay, respectively.
Conclusions:
LMs with a mean diameter between 1.1 and 2.6 mm induced significant hepatic perfusion changes, detected by CT. Such detection may help to select patients and propose chemotherapy at the time of primary tumor resection.
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