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    This summary is machine-generated.

    Partially coherent light offers advantages for microscopy, preventing speckle noise and crosstalk. This study introduces a non-interferometric system for quantitative phase imaging with simultaneous coherence determination.

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    Area of Science:

    • Optics and Photonics
    • Microscopy Techniques
    • Image Processing

    Background:

    • Partially coherent light offers advantages over completely coherent light in imaging.
    • Coherent light can cause speckle noise and cross-talk, degrading image quality.
    • Accurate quantitative imaging in microscopy benefits from partially coherent illumination.

    Purpose of the Study:

    • To present a non-interferometric technique for quantitative phase imaging.
    • To develop a system for simultaneous determination of illumination spatial coherence properties.
    • To demonstrate the practical benefits of partial coherence in imaging applications.

    Main Methods:

    • A non-interferometric quantitative phase imaging system was developed.
    • The system incorporates an electrically tunable lens and an sCMOS camera.
    • Simultaneous determination of spatial coherence properties was achieved.
    • High-speed measurements in the millisecond range were enabled.

    Main Results:

    • The performance of the technique was experimentally demonstrated.
    • Benefits of partial coherence for practical imaging were underlined.
    • The system allows for accurate quantitative phase imaging.
    • Speckle noise and cross-talk were mitigated.

    Conclusions:

    • The presented non-interferometric technique effectively utilizes partially coherent light for quantitative phase imaging.
    • Simultaneous coherence determination enhances imaging accuracy and practical applicability.
    • The developed system offers high-speed, millisecond-range measurements, beneficial for dynamic samples.