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Updated: Apr 28, 2026

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
Published on: June 24, 2013
Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of
Abstract:
We present an optical holographic micro-tomographic technique for imaging both the three-dimensional structures and dynamics of biological cells. Optical light field images of a sample, illuminated by a plane wave with various illumination angles, are measured in a common-path interferometry, and thus both the three-dimensional refractive index tomogram and two-dimensional dynamics of live biological cells are measured with extremely high sensitivity. The applicability of the technique is demonstrated through quantitative and measurements of morphological, chemical, and mechanical parameters at the individual cell level.
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