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Electronically resonant third-order sum frequency generation spectroscopy using a nanosecond white-light
Optics Express
|June 13, 2014
Summary
We developed a new spectrometer for third-order sum frequency generation (TSFG) to analyze hemoproteins. This method reveals electronic states, offering potential for in vivo molecular imaging.
Area of Science:
- Nonlinear Optics
- Molecular Spectroscopy
- Biophysics
Background:
- Third-order sum frequency generation (TSFG) is a third-order nonlinear optical process.
- TSFG shares a generation mechanism analogous to third harmonic generation (THG).
- Broadband multiplex TSFG spectra can be obtained using a white-light supercontinuum.
Purpose of the Study:
- To develop an electronically resonant TSFG spectrometer.
- To apply the developed spectrometer for obtaining TSFG spectra of hemoproteins.
- To demonstrate the potential of TSFG for imaging electronic states within biological systems.
Main Methods:
- Development of an electronically resonant third-order sum frequency generation (TSFG) spectrometer.
- Utilizing a white-light supercontinuum for broadband spectral acquisition.
- Analysis of TSFG ratio spectra to identify resonant enhancements.
Main Results:
- Successfully obtained TSFG spectra of hemoproteins using the developed spectrometer.
- Analyzed TSFG ratio spectra demonstrated clear resonant enhancement.
- The observed enhancement is directly attributable to the electronic state of hemoproteins.
Conclusions:
- The electronically resonant TSFG spectrometer is effective for analyzing hemoprotein electronic states.
- TSFG spectroscopy shows promise for non-invasive imaging of molecular electronic states.
- This technique could enable imaging of molecules within living cells or tissues.
