Tomography by point source digital holographic microscopy

We propose a tomographic method for point source inline holographic microscopy. By recording a set of holograms at different illumination angles, shadowing effects are eliminated resulting in three-dimensional images with the same precision at the micrometer-scale in all directions. The advantage of our tomographic approach is that it works for both absorbing and phase objects, regardless of the change of refractive index at interfaces. We develop the method with computer simulations and demonstrate its strength by presenting experimental results for micrometer-sized polystyrene beads and a cotton fiber.