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Related Experiment Videos

Temperature-sensitive gel for virus concentration from urine.

S Maheshkumar1, R B Peterson, S M Goyal

  • 1Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.

Journal of Virological Methods
|January 1, 1989
PubMed
Summary
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Temperature-sensitive gels effectively concentrate viruses like bacteriophage T-2 from urine. This reusable gel technology offers a 53% recovery rate, aiding in virus concentration for research.

Area of Science:

  • Biotechnology
  • Virology
  • Materials Science

Background:

  • Concentrating viruses from biological samples is crucial for detection and research.
  • Conventional methods can be time-consuming and may lead to virus loss.
  • Developing efficient and reusable concentration techniques is an ongoing need.

Purpose of the Study:

  • To evaluate the efficacy of temperature-sensitive polyacrylamide gels for virus concentration.
  • To determine the recovery rates of bacteriophage T-2 and feline herpes virus using this method.

Main Methods:

  • Utilized cross-linked, partially hydrolyzed polyacrylamide gels that exhibit temperature-dependent swelling and collapsing properties.
  • Tested the gel's ability to concentrate bacteriophage T-2 from 50 ml urine samples.

Related Experiment Videos

  • Assessed virus recovery rates and gel reusability over multiple cycles.

    • Main Results:

    • Achieved an average virus recovery of 53% for bacteriophage T-2, reducing sample volume by approximately 10-fold.
    • Demonstrated a 6-fold volume decrease with 54% recovery for feline cell-associated herpes virus.
    • Confirmed that the temperature-sensitive gel maintained its efficiency after repeated use.

    Conclusions:

    • Temperature-sensitive gels provide an effective method for concentrating viruses from biological fluids.
    • The technology offers a promising, reusable, and efficient approach for virus concentration in laboratory settings.
    • This method has potential applications in diagnostics and virological research.