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Updated: Jan 26, 2026

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
Published on: October 13, 2021
Use of Temperature-Sensitive Gel for the Concentration of Escherichia Coli in Milk
S Maheshkumar1, Richard B Peterson1, Sagar M Goyal1
1Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, 1943 Carter Avenue, St. Paul, Minnesota 55108.
Abstract:
Temperature-sensitive gels (cross-linked, partially hydrolyzed polyacrylamide gels) with the property to absorb solutes less than 1 nm in diameter at 4°C and collapse at higher temperatures (190°C) were used to concentrate Escherichia coli from 2% low-fat milk. Milk samples seeded with bacteria were reduced eight-fold in volume with average recoveries of 40-66% at an optimal pH of 5.5. Repeated use of the gel did not affect its efficiency.
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