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Peroxidative events in stored platelet concentrates
1Blood Transfusion and Immunohematology Service, Catholic University S. Cuore, Rome, Italy.
Vox Sanguinis
|January 1, 1989
Summary
Platelet concentrates stored in gas-permeable bags show increased lipid peroxidation, leading to impaired platelet function. Metabolic activity is preserved, but aggregability decreases significantly during storage.
Area of Science:
- Biomedical Science
- Hematology
- Biochemistry
Background:
- Platelet concentrates are crucial for transfusions.
- Storage conditions affect platelet viability and function.
- Gas permeability of storage bags influences platelet metabolism.
Purpose of the Study:
- To investigate metabolic and functional changes in platelets stored in gas-permeable bags.
- To assess lipid peroxidation and antioxidant levels during storage.
- To correlate these changes with platelet aggregability.
Main Methods:
- Daily measurement of pH, gases (pO2, pCO2), bicarbonate, glucose, lactic acid, and lactic dehydrogenase.
- Assessment of platelet adenosine triphosphate (ATP) and aggregation induced by collagen and ADP.
- Quantification of lipid peroxidation markers (conjugated dienes, malonyldialdehyde) and antioxidants (coenzyme Q10, vitamin E).
Main Results:
- Increased pO2, conjugated dienes, malonyldialdehyde, lactic acid, and lactic dehydrogenase activity observed.
- Platelet ATP content remained stable, but aggregability significantly decreased.
- Decreased pCO2, cyclooxygenase activity, vitamin E, coenzyme Q10, bicarbonate, and glucose levels were noted.
Conclusions:
- Platelet metabolic activity appears preserved during storage.
- Increased lipid peroxidation correlates with impaired platelet function and reduced aggregability.
- Storage in gas-permeable bags impacts platelet quality, necessitating further investigation.