Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Validation of single-sample doubly labeled water method.

M D Webster1, W W Weathers

  • 1Department of Avian Sciences, University of California, Davis 95616.

The American Journal of Physiology
|February 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Climate change. Uncertainty and climate change assessments.

Science (New York, N.Y.)·2001
Same author

Thermoregulation in Antarctic fulmarine petrels.

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology·2001
Same author

Seasonal changes in energy and water use by verdins, Auriparus flaviceps.

The Journal of experimental biology·2000
Same author

Effects of Plasmodium relictum on the metabolic rate and body temperature in canaries (Serinus canarius).

The Journal of parasitology·1987
Same author

Temperature and humidity dynamics of cutaneous and respiratory evaporation in pigeons, Columba livia.

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology·1987
Same author

Effects of heat stress on day-old broiler chicks.

Poultry science·1984
Same journal

Blood coagulation in fish.

The American journal of physiology·2011
Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010
Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010
Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010
Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010
Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010
See all related articles

This study validates a simplified doubly labeled water method for measuring metabolic rate and water turnover in small birds. The single-sample approach accurately estimates energy expenditure and water flux in verdins.

Area of Science:

  • Zoology
  • Physiology
  • Ecology

Background:

  • The doubly labeled water method is a standard for measuring energy expenditure in free-living animals.
  • Previous methods required multiple samples, limiting application in small or difficult-to-capture species.
  • A single-sample variant could enhance the practicality of metabolic studies.

Purpose of the Study:

  • To experimentally validate a single-sample variant of the doubly labeled water method.
  • To assess its accuracy for measuring metabolic rate and water turnover in a small passerine bird.
  • To compare results with established techniques like the Haldane gravimetric method.

Main Methods:

  • Used a single-sample doubly labeled water technique on nine verdins (Auriparus flaviceps).

Related Experiment Videos

  • Measured carbon dioxide production using the Haldane gravimetric method for comparison.
  • Calculated water flux using both the single-sample and standard two-sample doubly labeled water techniques.

    • Main Results:

    • Single-sample doubly labeled water estimates for CO2 production differed by less than 0.5% from Haldane values.
    • Water flux calculated by the single-sample method showed a -1.5% average difference from the two-sample method.
    • The method demonstrated good agreement with established physiological measurements.

    Conclusions:

    • The single-sample doubly labeled water method is a valid and accurate approach for small birds.
    • This simplified technique can reliably measure metabolic rate and water turnover.
    • It offers a practical alternative for physiological studies in small avian species.