Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.4K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.4K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.0K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A fully-automated low-cost cardiac monolayer optical mapping robot.

Frontiers in cardiovascular medicine·2023
Same author

Pericyte Progenitor Coupling to the Emerging Endothelium During Vasculogenesis via Connexin 43.

Arteriosclerosis, thrombosis, and vascular biology·2022
Same author

Sox6 as a new modulator of renin expression in the kidney.

American journal of physiology. Renal physiology·2019
Same author

Induced cardiomyocyte maturation: Cardiac transcription factors are necessary but not sufficient.

PloS one·2019
Same author

Cardiomyocyte Maturation Requires TLR3 Activated Nuclear Factor Kappa B.

Stem cells (Dayton, Ohio)·2018
Same author

Demethylation of H3K27 Is Essential for the Induction of Direct Cardiac Reprogramming by miR Combo.

Circulation research·2017

Related Experiment Video

Updated: Apr 28, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

6.8K

Reprogramming approaches in cardiovascular regeneration.

Sophie Dal-Pra1, Maria Mirotsou

  • 1Division of Cardiology, Department of Medicine, Duke University Medical Center & Duke Cardiovascular Research Center, GSRBII, Room 4022, 210 Research Drive, Durham, NC, 27710, USA.

Current Treatment Options in Cardiovascular Medicine
|June 15, 2014
PubMed
Summary

Cell reprogramming offers a promising avenue for cardiac repair by generating essential heart cells. This overview explores current strategies for creating cardiomyocytes, endothelial cells, and smooth muscle cells for regenerative therapy.

More Related Videos

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

2.9K
Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

790

Related Experiment Videos

Last Updated: Apr 28, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

6.8K
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

2.9K
Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

790

Area of Science:

  • Cardiovascular research
  • Regenerative medicine
  • Cell biology

Background:

  • Cardiac repair after injury requires restoring blood vessels and heart muscle for adequate oxygen and nutrient supply.
  • Recent advancements in direct cell reprogramming suggest potential for cardiac regenerative therapy.
  • Generating functional cardiomyocytes, endothelial cells, and smooth muscle cells is crucial for myocardial regeneration.

Purpose of the Study:

  • To provide an overview of current cell reprogramming strategies for generating cardiomyocytes, endothelial cells, and smooth muscle cells.
  • To discuss the implications of these reprogramming methods for cardiac regeneration.
  • To identify challenges and limitations for future therapeutic development.

Main Methods:

  • Review of existing literature on cell reprogramming techniques.
  • Analysis of strategies for in vitro generation of cardiomyocytes (CMs), endothelial cells (ECs), and smooth muscle cells (SMCs).
  • Discussion of the potential applications and hurdles in translating these methods to clinical cardiac repair.

Main Results:

  • Cell reprogramming can generate various cell types needed for cardiac repair, including CMs, ECs, and SMCs.
  • The study outlines diverse reprogramming strategies currently being explored.
  • Key challenges and limitations for clinical application are identified.

Conclusions:

  • Cell reprogramming holds significant promise for cardiac regenerative therapy by enabling the generation of essential cardiac cell types.
  • Further research is needed to overcome existing challenges and limitations for effective clinical translation.
  • Optimizing reprogramming efficiency and ensuring functional integration of generated cells are critical for future therapies.