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Nuclear GTP-binding proteins of Swiss 3T3 cells
S Takeda1, H Sugiyama, S Natori
1Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
FEBS Letters
|February 27, 1989
Abstract:
The GTP-binding proteins of Swiss 3T3 cell nuclei were analyzed by filter binding assay and UV cross-linking analysis. The results showed the presence of multiple GTP-binding proteins in the nuclei. Scatchard analysis revealed that the Kd value for GTP binding to high-affinity components was 69 nM, that to low-affinity components being 2.7 microM. The GTP-binding activities of some nuclear proteins were found to change significantly in response to the growth conditions of the cells. During culture of cells in medium without serum, the GTP-binding activity of a 140 kDa protein clearly decreased, whereas that of a 40 kDa protein increased.