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Updated: Apr 28, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

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Downregulation of Notch modulators, tetraspanin 5 and 10, inhibits osteoclastogenesis in vitro

Jian Zhou1,2, Toshifumi Fujiwara2, Shiqiao Ye2

  • 1Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, P. R. China.

Calcified Tissue International
|June 18, 2014
PubMed

Insights

Two tetraspanins, Tspan-5 and Tspan-10, are crucial for osteoclast differentiation. Their knockdown impairs Notch signaling, highlighting their role in bone cell formation and skeletal development.

Area of Science:

  • Bone Biology
  • Cell Signaling
  • Molecular Genetics

Background:

  • Notch signaling is vital for skeletal development and bone remodeling, regulating osteoblast and osteoclast differentiation.
  • The specific factors and pathways controlling Notch activation within bone cells are not well understood.

Purpose of the Study:

  • To investigate the role of tetraspanins, specifically TspanC8 members, in regulating osteoclast differentiation and Notch signaling.
  • To identify novel regulators of Notch pathway activation in bone cells.

Main Methods:

  • In vitro study using cell cultures.
  • Osteoclast differentiation assays.
  • Knockdown of Tspan-5 and Tspan-10 expression using short hairpin RNAs (shRNAs).
  • Analysis of ADAM10 maturation and Notch activation levels.

Main Results:

  • Tspan-5 and Tspan-10 expression increases during osteoclast differentiation.
  • Knockdown of Tspan-5 and Tspan-10 significantly inhibits osteoclastogenesis.
  • Loss of Tspan-5 and Tspan-10 leads to reduced ADAM10 maturation and attenuated Notch activation.

Conclusions:

  • Tspan-5 and Tspan-10 are critical regulators of osteoclast formation.
  • These tetraspanins modulate Notch signaling, playing a key role in osteoclastogenesis.
  • The findings reveal a novel mechanism involving TspanC8 tetraspanins in bone cell regulation.

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