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A linkage map of the mouse immunoglobulin lambda light chain locus.
Immunogenetics
|January 1, 1989
Summary
Researchers mapped the mouse immunoglobulin lambda light chain locus, finding its gene segments arranged as V2-J2C2J4C4-V1-J3C3J1C1. This 300 kb locus spans approximately 70 kb between V and J-C segments, suggesting ancestral duplication.
Area of Science:
- Immunogenetics
- Molecular Biology
- Genomics
Background:
- The mouse immunoglobulin lambda light chain locus is crucial for adaptive immunity.
- Classical understanding includes two V and four J-C gene segments in inbred mouse strains.
Purpose of the Study:
- To physically map the mouse immunoglobulin lambda light chain locus.
- To determine the arrangement and size of the lambda light chain gene segments.
Main Methods:
- Field inversion gel electrophoresis was used for locus linkage.
- Methylation-sensitive restriction enzyme digests defined the locus size.
- Fine mapping was performed using specific rearranged cell lines.
Main Results:
- The lambda light chain locus spans approximately 300 kb.
- The gene segment order was confirmed as V2-J2C2J4C4-V1-J3C3J1C1.
- A gap of ~70 kb was identified between V and J-C segments in the lambda 1 cluster, with a similar distance in the lambda 2 cluster.
Conclusions:
- The physical map supports the proposed gene order.
- The similar inter-segment distances suggest duplication of an ancestral locus.
- No significant HTF islands were detected within the mapped region.