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Downregulation of caveolin-1 in chronic rhinosinusitis with and without nasal polyps

Hai Lin1, Dong Lin, Xi-Sheng Xiong

  • 1Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Xuhui District, Shanghai, 200031, China.

European Archives of Oto-Rhino-Laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
|June 20, 2014
PubMed

Insights

Chronic rhinosinusitis (CRS) shows reduced caveolin-1 (Cav-1) expression, impairing its role in host defense. This downregulation in CRS, particularly with nasal polyps, leads to increased cyclin D1 and eNOS levels.

Area of Science:

  • Immunology
  • Molecular Biology
  • Otorhinolaryngology

Background:

  • Chronic rhinosinusitis (CRS) pathogenesis is not fully understood.
  • Caveolin-1 (Cav-1) is a protein involved in host defense and tumor suppression.
  • The role of Cav-1 in CRS requires further investigation.

Purpose of the Study:

  • To investigate the expression and function of Cav-1 in patients with CRS.
  • To determine the relationship between Cav-1, cyclin D1, and endothelial nitric oxide-synthase (eNOS) in CRS.
  • To explore the regulatory effects of cytokines on Cav-1 expression in CRS.

Main Methods:

  • Immunohistochemistry to assess Cav-1 protein expression.
  • Real-time polymerase chain reaction (PCR) for mRNA analysis of Cav-1, cyclin D1, and eNOS.
  • Nasal explant cultures to evaluate Cav-1 response to cytokine stimulation.

Main Results:

  • Reduced Cav-1 protein and mRNA expression observed in CRS, especially in CRS with nasal polyps (CRSwNP).
  • Elevated mRNA levels of cyclin D1 and eNOS found in CRS patients.
  • Cav-1 expression negatively correlated with cyclin D1 and eNOS expression.
  • IFN-γ augmented Cav-1 mRNA, while IL-4 and IL-1β suppressed it.

Conclusions:

  • Cav-1 expression is downregulated in CRS, particularly in CRSwNP.
  • Impaired Cav-1 function contributes to CRS by attenuating inhibition of cyclin D1 and eNOS.
  • Overexpression of cyclin D1 and eNOS is a consequence of Cav-1 downregulation in CRS.
  • IFN-γ appears crucial for maintaining Cav-1 gene expression.

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