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Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag
1School of Life Science, University of Science and Technology of China, Huangshan Road 443, 230026, Hefei, Anhui, People's Republic of China.
Methods in Molecular Biology (Clifton, N.J.)
|June 20, 2014
Abstract:
Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.

