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Related Concept Videos

Probiotics01:22

Probiotics

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Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
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Functions of the Gut Microbiota01:18

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The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
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Mucosal Barrier of the Stomach01:25

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The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
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Multispecies probiotic protects gut barrier function in experimental models.

Mylene Nébot-Vivinus1, Cherryl Harkat1, Hanene Bzioueche1

  • 1Mylene Nébot-Vivinus, Hanene Bzioueche, Department of Immunology, Pole of Biology, CHU de Nice, 06103 Nice, France.

World Journal of Gastroenterology
|June 20, 2014
PubMed
Summary

The probiotic Lactibiane Tolerance (LT) strengthens the gut barrier and reduces hypersensitivity. This study shows LT protects against damage from LPS, stress, and IBS patient factors, improving epithelial barrier function.

Keywords:
HypersensitivityIntestinal epithelial barrier permeabilityIrritable bowel syndromeProbiotic

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Area of Science:

  • Gastroenterology
  • Microbiology
  • Immunology

Background:

  • Epithelial barrier dysfunction is implicated in irritable bowel syndrome (IBS) and visceral hypersensitivity.
  • Probiotics are explored for their potential to modulate gut barrier function and reduce inflammation.
  • Lactibiane Tolerance (LT) is a multispecies probiotic combination.

Purpose of the Study:

  • To investigate the in vitro and in vivo effects of the probiotic combination Lactibiane Tolerance (LT) on epithelial barrier function.
  • To assess LT's impact on visceral hypersensitivity models.
  • To explore the mechanisms underlying LT's effects, including tight-junction expression and TLR-4 signaling.

Main Methods:

  • In vitro studies used T84 cell monolayers to assess permeability changes induced by lipopolysaccharide (LPS) or IBS patient-derived conditioned media (IBS-CM), with or without LT.
  • In vivo studies in mice involved stress models (water avoidance stress - WAS) and colonic instillation of IBS fecal supernatant (FSN) to evaluate visceral sensitivity and epithelial permeability.
  • Epithelial barrier integrity markers (occludin), tight junctions, and Toll-like receptor 4 (TLR-4) expression were analyzed.

Main Results:

  • LT significantly prevented increased paracellular permeability induced by LPS and IBS-CM in vitro, demonstrating a dose-dependent effect.
  • In vivo, LT administration protected against stress-induced and IBS FSN-induced epithelial disruption and visceral hypersensitivity.
  • LT treatment enhanced occludin expression and down-regulated TLR-4 mediated responses in both in vitro and in vivo models.

Conclusions:

  • The probiotic combination Lactibiane Tolerance (LT) effectively protects the epithelial barrier from disruption caused by inflammatory stimuli, stress, and factors from IBS patients.
  • LT demonstrates a therapeutic potential in preventing and managing visceral hypersensitivity associated with gut barrier dysfunction.
  • LT modulates key pathways, including tight junction regulation and TLR-4 signaling, contributing to its barrier-protective effects.