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Updated: Apr 28, 2026
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
Published on: January 28, 2017
Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas
Dan-Dan Yin1, Liang-Hui You1, Qing-Xin Yuan1
1Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
Aim:
To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.
Methods:
Overexpression or downregulation of mesothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro. Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.
Results:
Exogenous overexpression of mesothelin promoted cell proliferation, cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells. Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group. After an injection of adenovirus-mesothelin, a significantly increased number of small islets appeared, and the expression of PCNA was decreased on day 7 and day 14 compared with the Ad-EGFP group.
Conclusion:
Mesothelin was able to promote β cell proliferation in the remodeling stage of neonatal rats. Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.
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