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Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model
Published on: June 18, 2013
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Pathologic evaluation of normal and perfused term placental tissue
Lisa Leth Maroun1, Line Mathiesen, Morten Hedegaard
11 Department of Pathology, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark.
Summary
This study evaluated placental morphology in perfusion models, identifying trophoblastic vacuolization as a reproducible marker for fetal leakage. Pathologic examination improves placental perfusion models and requires precise definitions for common lesions.
Area of Science:
- Pathology
- Reproductive Biology
- Perfusion Science
Background:
- Histologic evaluation of placental tissue presents challenges in distinguishing normal tissue from pathologic lesions.
- Standardized criteria are needed for accurate assessment in placental research.
Purpose of the Study:
- To determine the incidence and interobserver variation of morphologic findings in term placentas used for perfusion studies.
- To develop and validate a scoring system for abnormal morphologic findings.
- To assess the utility of pathologic examination in placental perfusion models.
Main Methods:
- Light microscopic examination of 34 term placentas by two independent pathologists.
- Development of a scoring system for abnormal morphologic findings.
- Comparison of findings in normal and perfused tissues, correlating with perfusion success parameters.
Main Results:
- Trophoblastic vacuolization in perfused tissue was a reproducible finding correlated with fetal leakage.
- Bacteria were identified in fetal vessels of perfused placentas, leading to protocol adjustments (antibiotic addition).
- Revised criteria for frequent placental lesions improved interobserver agreement.
Conclusions:
- Pathologic examination is valuable for placental perfusion models.
- Trophoblastic vacuolization serves as a quality marker in perfusion studies.
- Clear definitions of placental abnormalities are essential for reproducible research.
