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Updated: Apr 27, 2026
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Published on: June 27, 2025
Genome-wide identification and functional prediction of novel and drought-responsive lincRNAs in Populus trichocarpa
Peng Shuai1, Dan Liang1, Sha Tang1
1College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, mailbox 69, No. 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China.
Abstract:
Protein-coding genes are considered to be a dominant component of the eukaryotic transcriptome; however, many studies have shown that intergenic, non-coding transcripts also play an important role. Long intergenic non-coding RNAs (lincRNAs) were found to play a vital role in human and Arabidopsis. However, lincRNAs and their regulatory roles remain poorly characterized in woody plants, especially Populus trichocarpa (P. trichocarpa). A large set of Populus RNA-Seq data were examined with high sequencing depth under control and drought conditions and a total of 2542 lincRNA candidates were identified. In total, 51 lincRNAs and 20 lincRNAs were identified as putative targets and target mimics of known Populus miRNAs, respectively. A total of 504 lincRNAs were found to be drought responsive, eight of which were confirmed by RT-qPCR. These findings provide a comprehensive view of Populus lincRNAs, which will enable in-depth functional analysis.
