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Related Concept Videos

Sample Handling01:02

Sample Handling

3.0K
Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results.
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...
3.0K
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

1.6K
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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Colloidal precipitates01:09

Colloidal precipitates

5.7K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
5.7K
Fixation and Sectioning01:03

Fixation and Sectioning

6.0K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
6.0K
Precipitation Processes01:12

Precipitation Processes

5.0K
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
5.0K
Washing, Drying, and Ignition of Precipitates00:52

Washing, Drying, and Ignition of Precipitates

5.7K
After filtration, the precipitate is washed to remove coprecipitated impurities and any remaining mother liquor. Colloidal precipitates, such as silver chloride, are washed with an electrolyte (such as dilute nitric acid) to prevent the peptization of the precipitate. In the case of slightly soluble precipitates, the wash solution contains a common ion to reduce solubility. Lead sulfate, which is slightly soluble in water, is washed with dilute sulfuric acid. Similarly, wash solutions may be...
5.7K

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Updated: Apr 27, 2026

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
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Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Published on: April 12, 2018

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Additives to preservation solutions.

T Saint Yves1, P-O Delpech1, S Giraud2

  • 1Inserm U1082, université de Poitiers, CHU La Miletrie, 86000 Poitiers, France; Service d'Urologie, CHU La Miletrie, 86000 Poitiers, France.

Progres En Urologie : Journal De L'Association Francaise D'Urologie Et De La Societe Francaise D'Urologie
|June 22, 2014
PubMed
Summary
This summary is machine-generated.

Improving organ preservation requires understanding ischemia reperfusion (IR) injury mechanisms. This study analyzes molecular interventions for better graft outcomes by examining cellular and systemic effects during preservation.

Keywords:
AdditivesIschémia reperfusionIschémie-reperfusionKidney transplantationPreservation solutionSolution de préservationSupplementsTransplantation rénale

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Area of Science:

  • Transplantation
  • Organ Preservation
  • Ischemia Reperfusion Injury

Background:

  • Ischemia reperfusion (IR) injury significantly impacts organ graft survival.
  • Current preservation techniques aim to minimize IR lesions.
  • Pharmacological supplements offer a promising strategy to enhance graft quality.

Purpose of the Study:

  • To evaluate the benefits of various molecular interventions against IR injury.
  • To provide a mechanistic understanding of molecular effects at cellular and systemic levels.
  • To guide the development of synergistic pharmaceutical regimens for improved organ preservation.

Main Methods:

  • Review of studies demonstrating molecular benefits against IR.
  • Thorough mechanistic analysis of molecular actions during preservation.
  • Assessment of effects at both cellular and systemic levels.

Main Results:

  • Several molecules show potential benefits in mitigating IR injury.
  • Mechanistic insights are crucial for selecting effective pharmaceutical interventions.
  • A comprehensive approach is needed to understand synergistic effects.

Conclusions:

  • Understanding the mechanisms of IR injury is key to improving organ preservation.
  • Targeted molecular interventions, analyzed mechanistically, can enhance graft outcomes.
  • Developing synergistic pharmaceutical strategies holds promise for reducing IR lesions and improving transplantation success.