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Updated: Apr 27, 2026

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
Biological effects of individually synthesized TNF-binding domain of variola virus CrmB protein
D D Tsyrendorzhiev1, I A Orlovskaya, S V Sennikov
1Research Institute of Clinical Immunology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, tsdon@mail.ru.
Abstract:
The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the efficiency of its inhibition of TNF cytotoxicity towards L929 mouse fibroblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.
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