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Related Experiment Videos

Improved lung preservation using a dimethylthiourea flush.

D E Paull1, B A Keagy, E J Kron

  • 1Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill 27599.

The Journal of Surgical Research
|April 1, 1989
PubMed
Summary
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Dimethylthiourea (DMTU) improves lung preservation for transplantation by reducing reperfusion injury. This hydroxyl free radical scavenger decreased edema and lipid peroxidation in canine lung lobes, enhancing organ viability.

Area of Science:

  • Transplantation Science
  • Organ Preservation
  • Free Radical Scavenging

Background:

  • Hypothermic preservation is critical for lung transplantation.
  • Reperfusion injury can compromise lung graft function.
  • Hydroxyl free radicals contribute to cellular damage during ischemia-reperfusion.

Purpose of the Study:

  • To evaluate dimethylthiourea (DMTU) as a hydroxyl free radical scavenger for improving lung preservation.
  • To assess the impact of DMTU on lung edema and lipid peroxidation post-preservation.

Main Methods:

  • Isolated canine left lower lobes were preserved using Euro-Collins solution with or without 20 mM DMTU for 4 hours at 4°C.
  • Lobes were reperfused with autologous blood for 90 minutes.
  • Capillary permeability coefficient (Kfc) and extravascular lung water were measured using the isogravimetric method.

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  • Lipid peroxidation was quantified as thiobarbituric acid-reactive material.

    • Main Results:

    • DMTU-enhanced preservation significantly reduced capillary permeability (Kfc) and extravascular lung water compared to controls.
    • Lipid peroxidation was significantly lower in DMTU-treated lungs (91 nmole/g) versus untreated controls (146 nmole/g).
    • Kfc values were 0.10 (control), 0.17 (Euro-Collins), and 0.10 ml/min/mm Hg/100 g lung (DMTU).

    Conclusions:

    • Dimethylthiourea (DMTU) effectively improves hypothermic lung preservation for transplantation.
    • DMTU reduces lung edema and lipid peroxidation upon reperfusion, mitigating injury.
    • This hydroxyl free radical scavenger shows promise for enhancing lung graft viability.