Related Experiment Video

Updated: Apr 27, 2026

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
10:38

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

9.6K

Remodeling of channel-forming ORAI proteins determines an oncogenic switch in prostate cancer

Charlotte Dubois1, Fabien Vanden Abeele1, V'yacheslav Lehen'kyi1

  • 1Inserm U1003, Laboratory of Excellence, Ion Channels Science and Therapeutics, Equipe Labellisée par la Ligue Nationale Contre le Cancer, SIRIC ONCOLille, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq 59656, France.

Cancer Cell
|June 24, 2014
PubMed

Insights

Altered ORAI channel protein levels in prostate cancer promote cell proliferation by forming novel ORAI3-ORAI1 channels. This disruption in calcium channel dynamics contributes to cancer development.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Ion Channel Physiology

Background:

  • ORAI family channels are implicated in cancer progression.
  • The specific mechanisms by which ORAI proteins reprogram cancer cells are not fully understood.

Purpose of the Study:

  • To investigate the role of ORAI protein expression levels in prostate cancer.
  • To elucidate how ORAI protein remodeling influences cancer cell behavior.

Main Methods:

  • Comparative analysis of ORAI protein expression in cancerous versus noncancerous prostate tissue.
  • In vitro modeling to study the functional consequences of ORAI protein remodeling.

Main Results:

  • Significant differences in ORAI protein expression were observed between prostate cancer and noncancerous tissues.
  • Enhanced ORAI3 expression led to heteromerization with ORAI1, forming novel channels.
  • These novel channels facilitated store-independent calcium entry, promoting cell proliferation.
  • A reduction in functional ORAI1 homomeric channels was noted, impacting apoptosis susceptibility.

Conclusions:

  • Disrupted dynamic equilibrium of ORAI channel-forming proteins represents a novel oncogenic mechanism in prostate cancer.
  • Targeting ORAI channel remodeling could offer therapeutic strategies for prostate cancer.

More Related Videos

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer08:54

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Published on: June 13, 2019

10.4K
A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer07:25

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

13.4K

Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.9K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

5.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

1.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
5.7K