Related Experiment Video
Updated: Apr 27, 2026

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
[Chemical constituents from the roots of Ilex pubescens]
Objective:
To study the chemical constituents from the roots of Ilex pubescens.
Methods:
The 95% ethanol extract of the plant was separated by silica gel, Sephadex LH-20 and preparative HPLC chromatography. The structures were elucidated based on the physiochemical properties and spectroscopic analysis.
Results:
Fifteen compounds were isolated and identified as oleuropein(1), oleoside dimethyl ester(2),8 (Z)-nuezhenide(3),3,4-dicaffeoylquinic acid methyl ester(4),3,4,5-tricaffeoylquinic acid methyl ester (5), isovanillic acid(6), syringic acid(7), 3beta-acetyloleanolic acid (8), 3beta-acetylursolic acid(9), uvaol(10), asiatic acid(11), 2alpha-hydroxyursolic acid(12), oleanolic acid (13), ursolic acid (14), stigmasterol-3-O-beta-D-glucopyranoside (15).
Conclusion:
Compounds 2,3,8,9 are obtained from this genus for the first time,compounds 5-7,10-12 are isolated from this plant for the first time.
More Related Videos
Related Concept Videos
Basic Plant Anatomy: Roots, Stems, and Leaves
Ion-Exchange Chromatography
Renewal of Intestinal Stem Cells
Basicity of Heterocyclic Aromatic Amines
IUPAC Nomenclature of Carboxylic Acids
For acyclic saturated monocarboxylic acids, the longest hydrocarbon chain containing the –COOH carbon is identified as the parent chain. Then, the last -e of the parent hydrocarbon name is replaced with a suffix -oic acid.

