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Updated: Apr 27, 2026
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Quantitative analysis of six flavonoids of hongzhu capsule by QAMS]
Objective:
To establish a method of quality assurance for mass-screening (QAMS) for simultaneously determining six flavonoids in Hongzhu Capsules.
Methods:
An HPLC (high performance liquid chromatography) method was developed as QAMS to determine neoeriocitrin, prunin, naringin, rhoifolin, miliditin and naringenin in Hongzhu Capsules. Using naringin as the internal reference substance, relative correction factors (RCF) of the five flavonoids were determined and given as follows (1.05, 0.782, 1.89, 1.27, 0.532). The contents of the flavonoids in Hongzhu Capsules were determined by QAMS and validated by the external standard method.
Results:
RCF values were determined by HPLC with good reproducibility. No significant difference between the quantitative results of QAMS and external standard method was observed.
Conclusion:
The present-developed method is convenient and accurate to determine multiple components when some standard substances are unavailable, thus considered as a potential method for quality control of Hongzhu Capsules.
