Related Experiment Video
Updated: Apr 27, 2026
07:16
In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry
Published on: April 5, 2015
9.6K
Quantitative buffy coat analysis-an effective tool for diagnosing blood parasites
Nishat Hussain Ahmed1, Jyotish Chandra Samantaray2
1Assistant Professor, Department of Laboratory Medicine, Delhi State Cancer Institute , Dilshad Garden, Delhi-110095, India .
Journal of Clinical and Diagnostic Research : JCDR
|June 25, 2014
Summary
Quantitative Buffy Coat (QBC) analysis is a sensitive and specific method for detecting malaria parasites and diagnosing filariasis. It also aids in diagnosing difficult cases of visceral Leishmaniasis.
Area of Science:
- Medical diagnostics
- Parasitology
- Hematology
Background:
- Quantitative Buffy Coat (QBC) analysis utilizes centrifugal stratification of blood components.
- It is a recognized sensitive technique for detecting peripheral blood parasites.
Purpose of the Study:
- To evaluate the efficacy of QBC analysis for malaria parasite detection and speciation.
- To assess QBC analysis as a diagnostic tool for filariasis.
- To explore the utility of QBC cellular patterns in diagnosing visceral Leishmaniasis.
Main Methods:
- Blood samples were analyzed using QBC analysis.
- Parasite detection, speciation, and cellular patterns were examined.
Main Results:
- QBC analysis demonstrated high sensitivity and specificity for malaria parasite detection and speciation in the Indian context.
- The technique proved sensitive and specific for diagnosing filariasis.
- Buffy coat cellular patterns aided in diagnosing challenging visceral Leishmaniasis cases.
Conclusions:
- QBC analysis is a versatile and effective diagnostic tool for malaria, filariasis, and visceral Leishmaniasis.
- Its application extends to aiding diagnosis in complex parasitic infections.
