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Localization of the replication origin of plasmid pE194
1Department of Microbiology, Public Health Research Institute, New York, New York 10016.
Journal of Bacteriology
|May 1, 1989
Abstract:
The pE194 replication origin was localized to a 265-base-pair interval by analyzing the ability of purified pE194 restriction fragments to direct replication of heterologous plasmids. Replication was dependent upon RepF protein supplied in trans. The origin region contained a GC-rich dyad symmetry which may serve as the RepF target.