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An Isolated Working Heart System for Large Animal Models
Published on: June 11, 2014
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An isolated working heart system for large animal models.
Matthew A Schechter1, Kevin W Southerland1, Bryan J Feger1
1Department of Surgery, Duke University Medical Center.
Journal of Visualized Experiments : Jove
|June 26, 2014
Summary
This study presents an improved Langendorff isolated heart perfusion apparatus using a centrifugal pump. This innovation allows for precise perfusion pressure control, enabling studies across various mammalian heart sizes and improving cardiovascular research.
Area of Science:
- Physiology
- Cardiovascular Research
- Biomedical Engineering
Background:
- The Langendorff apparatus is crucial for studying heart function and disease.
- Current limitations include difficulty regulating perfusion pressure for diverse heart sizes.
- This necessitates using primarily small animal models.
Purpose of the Study:
- To describe an adaptable Langendorff working heart apparatus.
- To overcome limitations in perfusing various mammalian heart sizes.
- To enhance cardiovascular research flexibility and data acquisition.
Main Methods:
- Replaced the traditional hydrostatic afterload column with a centrifugal pump.
- Enabled seamless switching between constant pressure and constant flow during reperfusion.
- Maintained an open system for effluent collection and pressure-volume data.
Main Results:
- Achieved easy adjustment and tight regulation of perfusion pressures.
- Demonstrated suitability for a range of mammalian heart sizes.
- Facilitated straightforward collection of ventricular pressure-volume data.
Conclusions:
- The modified apparatus offers enhanced versatility for cardiovascular research.
- This improved system supports studies across different species and heart sizes.
- It provides a valuable tool for advancing the understanding of heart function and disease.
