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Updated: Apr 27, 2026

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
Published on: May 14, 2020
Non-coding RNA transcription and RNA-directed DNA methylation in Arabidopsis
Xin-Jian He1, Ze-Yang Ma1, Zhang-Wei Liu1
1National Institute of Biological Sciences, Beijing 102206, China.
Abstract:
RNA-directed DNA methylation (RdDM) is responsible for transcriptional silencing of endogenous transposable elements and introduced transgenes. This process requires non-coding RNAs produced by DNA-dependent RNA polymerases IV and V (Pol IV and Pol V). Pol IV-produced non-coding RNAs are precursors of 24-nt small interfering RNAs, whereas Pol V-produced ncRNAs directly act as scaffold RNAs. In this review, we summarize recent advances in the understanding of RdDM. In particular, we focus on the mechanisms underlying the recruitment of Pol IV and Pol V to chromatin and the targeting of RdDM.
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