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Quantifying the Antifungal Activity of Peptides Against Candida albicans
Published on: January 13, 2023
Antimicrobial activity of cationic peptides in endodontic procedures
Sofi Beaula Winfred1, Gowri Meiyazagan1, Jiban J Panda2
1Department of Human Genetics, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, India.
Objectives:
The present study aimed to investigate the antimicrobial and biofilm inhibition activity of synthetic antimicrobial peptides (AMPs) against microbes such as Enterococcus faecalis, Staphylococcus aureus, and Candida albicans which are involved in endodontic infections.
Materials And Methods:
Agar diffusion test was done to determine the activity of peptides. The morphological changes in E. faecalis and reduction in biofilm formation after treatment with peptides were observed using scanning electron microscope. The efficacy of peptides using an ex vivo dentinal model was determined by polymerase chain reaction and confocal laser scanning microscopy. Platelet aggregation was done to determine the biocompatibility of peptides.
Results:
Among 11 peptides, two of the amphipathic cationic peptides were found to be highly active against E. faecalis, S. aureus, C. albicans. Efficacy results using dentinal tubule model showed significant reduction in microbial load at 400 μm depth. The peptides were also biocompatible.
Conclusion:
These results suggest that synthetic AMPs have the potential to be developed as antibacterial agents against microorganisms involved in dental infections and thus could prevent the spread and persistence of endodontic infections improving treatment outcomes and teeth preservation.
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