Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pleiotropy01:33

Pleiotropy

31.2K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
31.2K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

6.8K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
6.8K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K
Production of Formed Elements01:34

Production of Formed Elements

7.3K
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
7.3K
Primary Lymphoid Organs01:16

Primary Lymphoid Organs

9.6K
Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
9.6K
Lineage Commitment01:21

Lineage Commitment

3.4K
Commitment is the  process whereby stem cells:
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and function of specified thymic iNKT1 cells critically depend on an Ets1-Tbet transcription factor axis.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Transcriptional control of natural killer cell antitumor activity.

Trends in immunology·2025
Same author

Loss of thymocyte competition underlies the tumor suppressive functions of the E2a transcription factor in T-ALL.

Leukemia·2023
Same author

TGF-β Promotes the Postselection Thymic Development and Peripheral Function of IFN-γ-Producing Invariant NKT cells.

Journal of immunology (Baltimore, Md. : 1950)·2023
Same author

Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic Leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research·2023
Same author

Quantitative control of Ets1 dosage by a multi-enhancer hub promotes Th1 cell differentiation and protects from allergic inflammation.

Immunity·2023
Same journal

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
Same journal

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same journal

EBV and immunodeficiency: the odd couple drawn to the brain.

Blood·2026
Same journal

A bone to pick with ferric carboxymaltose.

Blood·2026
Same journal

A step toward streamlining HIT diagnosis.

Blood·2026
See all related articles

Related Experiment Video

Updated: Apr 27, 2026

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
08:59

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Published on: July 17, 2017

12.5K

Sox4 B-lymphocyte progenitors.

Barbara L Kee1

  • 1THE UNIVERSITY OF CHICAGO.

Blood
|June 28, 2014
PubMed
Summary
This summary is machine-generated.

This study reveals the crucial role of the Sox4 transcription factor in pro-B lymphocyte development. Researchers used genetic manipulation and gene expression analysis to understand its function.

More Related Videos

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Published on: April 7, 2008

39.7K
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
12:17

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Published on: January 2, 2016

7.9K

Related Experiment Videos

Last Updated: Apr 27, 2026

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
08:59

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Published on: July 17, 2017

12.5K
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Published on: April 7, 2008

39.7K
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
12:17

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Published on: January 2, 2016

7.9K

Area of Science:

  • Hematology
  • Molecular Biology
  • Immunology

Background:

  • The development of B lymphocytes is a complex process involving precise regulation of gene expression.
  • Transcription factors play critical roles in orchestrating cellular differentiation pathways.
  • Sox4 is a transcription factor implicated in various developmental processes, but its specific functions in early B-cell development require further elucidation.

Purpose of the Study:

  • To investigate the mechanistic functions of the transcription factor Sox4 in pro-B lymphocytes.
  • To understand the regulatory network controlled by Sox4 during early B-cell development.

Main Methods:

  • Utilized gain-of-function and loss-of-function approaches to manipulate Sox4 levels in pro-B lymphocytes.
  • Performed global gene expression analysis (e.g., RNA-sequencing) to identify Sox4-regulated genes.
  • Conducted genome-wide transcription factor binding analysis (e.g., ChIP-sequencing) to map Sox4 binding sites.

Main Results:

  • Sox4 significantly influences the expression of key genes involved in pro-B lymphocyte proliferation and survival.
  • Loss of Sox4 function leads to impaired pro-B cell development, characterized by reduced cell numbers and altered differentiation.
  • Gain of Sox4 function promotes pro-B cell expansion and affects specific developmental checkpoints.

Conclusions:

  • Sox4 is a critical regulator of pro-B lymphocyte development, controlling essential cellular processes.
  • The findings provide mechanistic insights into the transcriptional network governing early B-cell maturation.
  • Sox4 represents a potential therapeutic target for modulating B-cell malignancies.