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Updated: Apr 27, 2026
Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery
Published on: September 27, 2021
Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice
Malika Serra-Hassoun1, Maryline Bourgine2, Michele Boniotto3
1Unité de Biologie des Populations Lymphocytaires, Département d'Immunologie, Institut Pasteur, 75724 Paris, France; Centre National pour la Recherche Scientifique, Unité de Recherche Associée 1961, 75724 Paris, France;
Abstract:
We generated a new humanized mouse model to study HLA-restricted immune responses. For this purpose, we created unique murine hosts by enforcing the expression of human SIRPα by murine phagocytes in murine MHC-deficient HLA-transgenic alymphoid hosts, an approach that allowed the immune reconstitution of nonpermissive mice following injection of human hematopoietic stem cells. We showed that these mouse/human chimeras were able to generate HLA-restricted responses to immunization. These new humanized mice may offer attractive models to study immune responses to human diseases, such as HIV and EBV infections, as well as to assay new vaccine strategies.
