Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting the cell membrane in established and emerging model organisms.

Development (Cambridge, England)·2026
Same author

Visualizing the multidimensional landscape of biological variation in modern microscopy.

Frontiers in bioinformatics·2026
Same author

Targeting the cell membrane in established and emerging model organisms.

bioRxiv : the preprint server for biology·2025
Same author

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue.

Nature biotechnology·2025
Same author

Seeing the heart in 3-D: Mapping arrhythmias across modalities.

The Journal of physiology·2025
Same author

T-CLEARE: a pilot community-driven tissue clearing protocol repository.

Frontiers in bioengineering and biotechnology·2024
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Related Experiment Video

Updated: Apr 27, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.4K

Light sheet microscopy.

Michael Weber1, Michaela Mickoleit1, Jan Huisken1

  • 1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.

Methods in Cell Biology
|June 30, 2014
PubMed
Summary
This summary is machine-generated.

This chapter details light sheet microscopy, an advanced imaging technique. It offers high-speed acquisition and minimal phototoxicity, making it a powerful alternative to confocal microscopy for biological research.

Keywords:
Developmental biologyFluorescence microscopyImage processingLight sheet microscopyMountingSelective plane illumination microscopyThree-dimensional imagingUltramicroscopyZebrafish

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.1K
Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
08:37

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Published on: May 5, 2014

25.9K

Related Experiment Videos

Last Updated: Apr 27, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.4K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.1K
Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
08:37

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Published on: May 5, 2014

25.9K

Area of Science:

  • Microscopy
  • Biophotonics
  • Optical Imaging

Background:

  • Confocal microscopy faces limitations in speed and phototoxicity for dynamic biological samples.
  • Light sheet microscopy offers advantages for high-resolution, long-term imaging.
  • Developing custom light sheet instruments requires specific design considerations.

Purpose of the Study:

  • To introduce the principles and practical aspects of designing and building light sheet microscopes.
  • To highlight selective plane illumination microscopy (SPIM) as a superior alternative to confocal microscopy.
  • To provide guidance on sample mounting and data processing for time-lapse imaging.

Main Methods:

  • Detailed explanation of light sheet microscopy principles.
  • Practical advice on instrument design and construction.
  • Discussion of multiview sample rotation techniques.

Main Results:

  • Selective plane illumination microscopy (SPIM) enables high-speed, full-frame acquisition.
  • SPIM significantly reduces phototoxicity, preserving sample viability during imaging.
  • Successful applications and key concepts derived from a decade of experience are summarized.

Conclusions:

  • Light sheet microscopy, particularly SPIM, is a valuable tool for advanced biological imaging.
  • Careful consideration of sample mounting and data processing is crucial for successful long-term time-lapse studies.
  • This work provides a practical guide for researchers interested in implementing light sheet microscopy.