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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

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Immune cell imaging using multi-spectral optoacoustic tomography.

Stratis Tzoumas, Angelika Zaremba, Uwe Klemm

|July 1, 2014

Summary

Multispectral optoacoustic tomography (MSOT) can image fluorescently labeled cells deep within tissues. This study quantifies MSOT

Area of Science:

Biomedical Imaging
Cellular Biology
Optoacoustics

Background:

Multispectral optoacoustic tomography (MSOT) enables high-resolution imaging of optically labeled cells.
Spectral unmixing in MSOT differentiates labeled cells from background tissue absorption, allowing deeper imaging than optical microscopy.
The relationship between spectrally resolved fluorescently labeled cells and optoacoustic detection requires systematic investigation.

Purpose of the Study:

To investigate the relationship between spectrally resolved fluorescently labeled cells and optoacoustic detection.
To establish the optoacoustic signal generated by fluorescently labeled cells as a function of cell number and cell type.
To assess the sensitivity of MSOT for resolving cells implanted in vivo.

Main Methods:

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Titration experiments were performed using fluorescently labeled cells.
Optoacoustic signals were measured as a function of cell number and across different cell types.
MSOT sensitivity was evaluated by imaging cells implanted in animal models.

Main Results:

The study established a quantifiable relationship between the number of fluorescently labeled cells and the generated optoacoustic signal.
Optoacoustic signal intensity varied across different cell types, indicating cell-specific detection capabilities.
MSOT demonstrated sensitivity in resolving implanted cells within animal models.

Conclusions:

MSOT provides a sensitive and quantitative method for imaging fluorescently labeled cells in vivo.
The findings support the use of MSOT for longitudinal cellular biology studies beyond the limits of optical microscopy.
Further research can optimize MSOT protocols for specific cell types and applications in preclinical research.