Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Toxicity Testing in Animals01:23

Toxicity Testing in Animals

200
Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
200

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted Delivery of mRNA to the Heart via Extracellular Vesicles or Lipid Nanoparticles.

Journal of extracellular vesicles·2026
Same author

Prospective germline exome and machine learning-based risk score identify predictive and prognostic biomarkers of immunotherapy outcomes in advanced non-small cell lung cancer.

Journal of translational medicine·2026
Same author

AI-driven quality control of cell-based ATMPs: automated, accurate, and affordable.

Cytotherapy·2026
Same author

Conventional type-1 DC density is associated with checkpoint inhibitor response across multiple types of cancer.

The Journal of clinical investigation·2026
Same author

A Long-Term Human Liver Spheroid Model for Assessing Silencing and Durability of GalNAc-Conjugated siRNAs.

Clinical and translational science·2026
Same author

Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma.

Oncoimmunology·2026

Related Experiment Video

Updated: Apr 27, 2026

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

47.0K

Long-term chronic toxicity testing using human pluripotent stem cell-derived hepatocytes.

Gustav Holmgren1, Anna-Karin Sjögren2, Isabel Barragan2

  • 1Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden (G.H., P.S., J.S.); Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden (G.H.); Department of Discovery Safety, Drug Safety and Metabolism, AstraZeneca R&D, Mölndal, Sweden (A.-K.S.); Section of Pharmacogenetics, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden (I.B., T.B.A., M.I.-S.); Department of Bioscience, Cardiovascular and Metabolic Diseases, AstraZeneca R&D, Mölndal, Sweden (A.S.); NovaHep AB, Gothenburg, Sweden (P.B.); Department of Drug Metabolism and Pharmacokinetics, AstraZeneca R&D, Mölndal, Sweden (T.B.A.); and Cellectis AB, Gothenburg, Sweden (P.S., P.B., J.E.) Gustav.holmgren@his.se.

Drug Metabolism and Disposition: the Biological Fate of Chemicals
|July 2, 2014
PubMed
Summary

Human pluripotent stem cells (hPSC)-derived hepatocytes show promise for long-term drug toxicity testing. These cells demonstrated increased sensitivity to toxic compounds and revealed specific liver damage, improving preclinical models.

More Related Videos

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

10.8K
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
06:57

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Published on: July 20, 2019

9.6K

Related Experiment Videos

Last Updated: Apr 27, 2026

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

47.0K
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

10.8K
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
06:57

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Published on: July 20, 2019

9.6K

Area of Science:

  • Hepatology
  • Toxicology
  • Stem Cell Biology

Background:

  • Current preclinical models for drug toxicity testing have limitations, particularly for chronic hepatotoxicity.
  • Late-stage pharmaceutical attrition is often due to non-predictive models lacking clinical relevance.
  • Human pluripotent stem cells (hPSC) offer potential for developing improved in vitro models.

Purpose of the Study:

  • To evaluate the utility of hPSC-derived hepatocytes for long-term in vitro drug toxicity assessment.
  • To investigate the response of hPSC-derived hepatocytes to prolonged exposure to hepatotoxic compounds.
  • To establish a more clinically relevant model for chronic liver toxicity studies.

Main Methods:

  • Human pluripotent stem cells were differentiated into hepatocytes.
  • Hepatocytes were subjected to repeated-dose incubation with hepatotoxic compounds for up to 14 days.
  • Cytotoxicity, phospholipidosis, and steatosis were assessed.

Main Results:

  • hPSC-derived hepatocytes exhibited increased sensitivity to toxic compounds with extended exposure.
  • Evidence of phospholipidosis and steatosis was observed, indicating specific liver damage.
  • This study represents the first report of a long-term toxicity study using hPSC-derived hepatocytes.

Conclusions:

  • hPSC-derived hepatocytes are a promising tool for long-term drug toxicity evaluation.
  • These models can provide more clinically relevant data than current preclinical systems.
  • Further development and validation of hPSC-based toxicity models are supported for evaluating drugs, chemicals, and cosmetics.