Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.1K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.1K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

8.2K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic Impact of Peripheral Expression of Mutant Huntingtin in <i>C. elegans</i>.

Journal of proteome research·2026
Same author

STIP1/HOP promotes the formation of cytotoxic α-synuclein oligomers.

Molecular neurodegeneration advances·2026
Same author

Toward Metabolomics Analyses With Combined Capillary Vibrating Sharp-Edge Spray Ionization and Atmospheric Pressure Chemical Ionization.

Rapid communications in mass spectrometry : RCM·2026
Same author

Examining the Impact of Side-Chain Chirality on Conformation of a Helical Poly(3‑(<i>S</i>‑1-ethylhexyl)esterfuran).

Macromolecules·2026
Same author

Exogenous Huntingtin-Exon1 Aggregates Exhibit Distinct Levels of Toxicity to <i>Caenorhabditis elegans</i>.

ACS chemical neuroscience·2025
Same author

Membranes as targets and modifiers of mutant huntingtin aggregation.

Trends in biochemical sciences·2025

Related Experiment Video

Updated: Apr 27, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

6.8K

Tapping, pulling, probing: atomic force microscopy in drug discovery.

Justin Legleiter1, Tomasz Kowalewski1

  • 1Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.

Drug Discovery Today. Technologies
|July 2, 2014
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) offers nanoscale imaging and mechanical property analysis in physiological conditions. This versatile tool is crucial for studying cellular and molecular interactions in drug discovery research.

More Related Videos

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

11.2K
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

8.5K

Related Experiment Videos

Last Updated: Apr 27, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

6.8K
Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

11.2K
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

8.5K

Area of Science:

  • Biophysics
  • Nanotechnology
  • Drug Discovery

Background:

  • Atomic Force Microscopy (AFM) is a high-resolution imaging technique.
  • AFM enables the study of mechanical properties and interaction forces at the nanoscale.
  • Operation in fluid media allows for experiments under physiological conditions.

Purpose of the Study:

  • To highlight the versatility of AFM in biological applications.
  • To emphasize AFM's utility in drug discovery.
  • To showcase AFM's capability in examining cellular and subcellular phenomena.

Main Methods:

  • Utilizing AFM for nanoscale surface imaging.
  • Employing AFM to probe local mechanical properties.
  • Measuring interaction forces using AFM.

Main Results:

  • AFM provides nanoscale resolution imaging of surfaces.
  • AFM can analyze mechanical properties and interaction forces.
  • Experiments can be performed under physiological conditions using AFM in fluids.

Conclusions:

  • AFM is a powerful tool for biological research due to its versatility and ability to operate in physiological conditions.
  • AFM facilitates the study of phenomena at the cellular and subcellular levels.
  • AFM offers unique opportunities in drug discovery for analyzing molecular complexes.