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Chondroitinase C activity of Streptococcus intermedius
1Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
FEMS Microbiology Letters
|January 15, 1989
Summary
Streptococcus intermedius ATCC 27335 produces an enzyme that breaks down chondroitin 6-sulfate. This specific depolymerizing activity indicates the presence of chondroitinase C, an enzyme crucial for glycosaminoglycan research.
Area of Science:
- Microbiology
- Biochemistry
- Enzymology
Background:
- Chondroitin sulfate is a vital glycosaminoglycan in connective tissues.
- Understanding enzymes that degrade chondroitin sulfate is important for biological research.
Purpose of the Study:
- To investigate the chondroitin sulfate depolymerizing activity in Streptococcus intermedius ATCC 27335 culture supernatant.
- To identify the specific enzyme responsible for this activity.
Main Methods:
- Culturing Streptococcus intermedius ATCC 27335.
- Analyzing the culture supernatant for enzymatic activity against chondroitin 6-sulfate.
- Testing enzyme activity against related substrates like chondroitin 4-sulfate and dermatan sulfate.
Main Results:
- The culture supernatant demonstrated depolymerizing activity specifically on chondroitin 6-sulfate.
- The enzyme cleaved 2-Acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose from the substrate.
- No activity was observed against chondroitin 4-sulfate or dermatan sulfate.
Conclusions:
- The observed depolymerization of chondroitin 6-sulfate is attributed to chondroitinase C.
- Streptococcus intermedius ATCC 27335 produces chondroitinase C, a specific enzyme for chondroitin 6-sulfate degradation.