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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Apr 27, 2026

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Phospho-specific flow cytometry in drug discovery.

Matthew R Clutter¹, Peter O Krutzik¹, Garry P Nolan¹

¹Baxter Laboratory of Genetic Pharmacology, Department of Microbiology and Immunology, School of Medicine, Stanford University, 269 Campus Drive, CCSR 3205, Stanford, CA 94305, USA.

Drug Discovery Today. Technologies

|July 2, 2014

Summary

Phospho-specific flow cytometry offers a novel tool for drug discovery, enhancing efficiency across the entire development pipeline. This high-content, high-throughput method aids in target identification, screening, and diagnostics.

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Area of Science:

Biotechnology
Immunology
Pharmacology

Background:

Flow cytometry is a powerful technique for analyzing single cells.
Drug discovery requires robust methods for target identification and validation.
Phospho-specific antibodies enable the study of signaling pathways.

Purpose of the Study:

To introduce phospho-specific flow cytometry as a versatile tool for drug discovery.
To highlight its applications across various stages of the drug development pipeline.
To demonstrate its potential for high-throughput screening and diagnostics.

Main Methods:

Utilizing phospho-specific antibodies for targeted protein analysis.
Applying flow cytometry for single-cell, multiparameter data acquisition.
Leveraging technological advancements for increased throughput capacity.

Main Results:

Demonstrated the utility of phospho-specific flow cytometry in drug discovery.
Showcased its applicability from target identification to clinical diagnostics.
Highlighted the generation of high-content datasets for comprehensive analysis.

Conclusions:

Phospho-specific flow cytometry is a valuable platform for modern drug discovery.
Its high-throughput capacity makes it suitable for large-scale screening.
The technique supports diverse applications, including disease modeling and diagnostics.